Mycobacterium avium subsp. paratuberculosis in a mouflon herd without clinical symptoms monitored using IS900 real-time PCR: a case report
نویسندگان
چکیده
منابع مشابه
Characterization of IS900 loci in mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing
Mycobacterium avium subsp. paratuberculosis is a pathogen that causes chronic inflammation of the intestine in many animals, including primates, and is implicated in Crohn's disease in humans. It differs from other members of the M. avium complex in having 14-18 copies of IS900 inserted into conserved loci in its genome. In the present study, genomic DNA flanking 14 of these insertions was char...
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Mycobacterium avium subsp. paratuberculosis (MAP) in concentrations from 48 to 32 500 cells per gram of powdered infant milk were found in 18 out of 51 investigated samples (35%) in this study. More than 10 000 cells per gram were present in four samples (7.8%). Such concentrations mean that one package of milk contains 5 million MAP cells, which are ingested by a bottle-fed baby over the cours...
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The study aimed at direct detection of Mycobacterium avium subsp. Paratuberculosis (MAP) in milk by evaluating a multiplex real-time PCR assay targeting IS900 and ISMAV2 sequences including the amplification of PUC19-plasmid as internal control. The sensitivity of the assays was evaluated by testing MAP isolates in broad linear range of DNA (50 ng – 5 fg/μl). For the validation of the specifici...
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Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows' milk in the United Kingdom and, potentially, elsewhere. Water supplies are also at risk. The ...
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ژورنال
عنوان ژورنال: Veterinární Medicína
سال: 2010
ISSN: 0375-8427,1805-9392
DOI: 10.17221/2943-vetmed